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Freezing stress is an important abiotic stress that limiting the yield and   the spatial distribution of many important crops. This study was undertaken   to screen 136 doubled haploid (DH) lines of camelina (Camelina sativa L.)   along with four canola (Brassica napus) cultivars (Hyola 401, Lord, Roska and   Cascade) as experimental control under freezing stress conditions (-14°C for   6 h) to identify lines with high or low level of tolerance to freezing stress   for further studies. First, a protocol was developed for large scale   screening of camelina germplasm under freezing stress conditions. For this   purpose, an experiment with different freezing temperatures (-5, -10, -15 and   -20°C) was conducted to find an appropriate temperature that discriminated   best between genotypes (i.e. the LT50 temperature). The LT50 values for   camelina lines were varied between -10.2 and -17.1°C with an average of   -13.94°C for all of the camelina lines. Therefore, we selected the -14°C   exposure for 6 h as an appropriate temperature to screening of camelina   lines. The principal components of measured parameters (LT50, survival   percentage, relative conductivity and scoring) was using principal component   analysis that determine freezing-tolerant and freezing-sensitive lines. Among   136 doubled haploid lines, some lines (58, 62 and 101) had higher level of   freezing tolerance and some of them (8, 16, 32, 91 and 107) were freezing   sensitive. The selected lines in a preliminary freezing screening are useful   for further evaluations

Screening of camelina (Camelina sativa L.) doubled haploid lines for freezing tolerance in the seedling stage