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The method of 16S-V3 rDNA PCR-DGGE was utilized to study the diversity of   methane-producing microbial community structure under different   methane-producing microbial community structure. Results showed that   community structure of the methanogens under different environmental   condition was significantly different. The number of bands about community   under extreme condition was less than normal conditions, but the main bands   were obvious. The sequence analysis showed that the predominant strains   mainly were uncultured bacteria that could not be identified in the level of   species .Z2 was Clostridia, Z8 was Clostridiales, D5 was Eubacteriaceae, and   D8 was Flavobacteriaceae. This study laid some extent foundation for future   research about establishing a methane-producing bacterial system with high   gas yield in winter

Diversity of methane-producing microbial community structure with 16S-V3 rDNA PCR-DGGE