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In this work, two real-time PCR protocols based on intercalating dye and two   on hydrolysis probes were tested using field collected fruit tree samples   infected by 16SrX group (AP, PD and ESFY) phytoplasmas. Specificity and   sensitivity of protocols and amplification efficiency were the main testing   parameters. Results of real-time PCR protocols were compared to nested PCR.   All real-time PCR protocols confirmed their specificity of detection. All   real-time PCR protocols were 10-100 times more sensitive than nested PCR.   Afterall real-time PCR protocols based on hydrolysis probes were 10 times   more sensitive than protocols based on intercalating dyes. Among protocols   based on hydrolysis probes, slightly better detection characteristics were   shown by protocol by CHRISTENSEN et al. (2004)

Comparison of real-time PCR protocols in detection and quantification of fruit tree 16SrX group phytoplasmas