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Plant steroidal hormones, brassinosteroids (BRs), promote plant developmental   processes and enhance tolerance to several abiotic stresses including high   boron (B) stress. To examine the possible role of BR in high B-induced stress   at the transcriptional level, we investigated the response of B transporter   genes (BOR1-4), high B-induced genes (MATE, Hsp-like), BR-induced genes   (Hsp70-4, Hsp90-1) and other stress-related genes (LTI/COR78, LEA4-5) upon   exogenous treatments of 24-epibrassinolide (EBL) on Arabidopsis thaliana (L.)   Heynh exposed to high concentrations of boric acid (BA) using   semi-quantitative RT-PCR. BA treatments led to down regulation of BOR1 and   BOR3 genes in leaf and root tissues and higher concentration of EBL further   decreased expression of these genes in roots. The expression of high   B-induced genes was observed to be upregulated by 1 μM EBL treatment under   high B stress in both tissues of the seedlings. The upregulation of   BR-induced genes were clearly evident in root tissues co-treated with 1 μM   EBL and BA as compared to BA alone. Higher concentration of EBL was found to   be more effective in increasing expression of LTI/COR78 gene in root and   LEA4-5 gene in shoot tissues. To our knowledge, this is the first report how   exogenous application of EBL modulates high B stress responses at molecular   level in model plant Arabidopsis thaliana

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Differential expression analysis of boron transporters and some stress-related genes in response to 24-epibrassinolide and boron by semi-quantitative RT-PCR in Arabidopsis thaliana (L.) Heynh