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Cirsium aduncum Fisch. & C.A.Mey. Ex DC. and C. haussknechtii Boiss.,   (Asteraceae) are important medicinal plant species that grow in different   geographical regions of Iran. We had no knowledge about population genetic   structure, intra-specific and inter-specific gene flow and the presence of   hybrid zone for this two species in Iran. Therefore, in order to provide data   for conservation of these two medicinally important species, the population   genetic analysis and morphometric studies were performed in 18 geographical   populations of these species. ANOVA and MDS analyses revealed significant   morphological difference among the studied populations in either species,   while MDS plot showed morphological overlap in plants of these two species.   AMOVA test revealed significant genetic difference among the studied   populations. Mantel test showed positive significant correlation between   genetic and geographical distances and the occurrence of isolation by   distance. Population assignment test and STRUCTURE plot of genetic data   revealed inter-specific introgression between these species

Contemporary inter-specific hybridization between Cirsium aduncum and C. haussknechtii (Asteraceae): Evidence from molecular and morphological data