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A somatic embryogenesis receptor kinase like (SERKL) cDNA, designated   PhSERKL, was isolated from date palm (Phoenix Dactylifera L) using RACE PCR.   PhSERKL protein shared all the characteristic domains of the SERK family,   including five leucine-rich repeats, one proline-rich region motif, a   transmembrane domain, and kinase domains. Phylogenetic analyses using PHYLIP   and Notung 2.7 programs suggest that the SERK proteins of some plant species   resulted from relatively ancient duplication events. We predict an ancestor   protein of monocots and dicots SERK using FASTML program. Somatic embryogenic   cultures of date palm were established following transfer of callus cultures   to medium containing 2, 4-dichlorophenoxyacetic acid. The role of PhSERKL   gene during establishment of somatic embryogenesis in culture was   investigated using quantitative real-time PCR. PhSERKL gene was highly   expressed during embryogenic competence acquisition and globular embryo   formation in culture. Overall, levels of expression of PhSERKL gene were   lower in nonembryogenic tissues and organs than in embryogenic callus

genetika

Molecular characterization and evolution studies of a SERK like gene transcriptionally induced during somatic embryogenesis in Phoenix Dactylifera L v Deglet Nour