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Random amplified polymorphic DNA (RAPD) markers were used to detect   polymorphism among accessions of wild sunflower species Hеlianthus   maximiliani, Helianthus tuberosus, Helianthus mollis and Helianthus rigidus   with different tolerance to mid-stalk white rot and selection of potential   markers for different levels of tolerance to this disease. Estimates of   genetic variation showed that genetic diversity was equally distributed   between Helianthus species and within them. Cluster analysis corresponded to   the phylogenetic relations within the genus Helianthus. The results obtained   by principal coordinates analysis (PCoA), where the first two principal   coordinates accounted for 83.7% of total variation, perfectly coincided with   the results of cluster analysis. Contingency coefficient significance test   showed that most of the used primers generated bands associated with some   level of tolerance or susceptibility to mid- stalk white rot. Furthermore,   contingency analysis showed that primer C12 generated bands associated with   resistance (100%) to mid-stalk white rot both in H. mollis and in all   accessions, while primer X18 generated bands significantly associated with   high tolerance (75%) in H. rigidus, H. mollis as well as in all tested   accessions. The C15-600 bp locus was found to be significantly associated   with high tolerance (75%) in all accessions, and medium tolerance (50%) in H.   mollis. [Projekat Ministarstva nauke Republike Srbije, br. TR 31025

Genetic diversity of wild sunflower (Helianthus sp.) accessions with different tolerance to mid-stalk white rot