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Linkage disequilibrium has been studied among three linked enzyme loci of   second chromosome of D. ananassae collected from five natural populations.   Each of the three enzyme loci, that is Acph1, Acph 2 and Xdh was represented   by two distinct alleles and in three genotypic forms. Thus nine genotypic   combinations were recorded for each enzyme pair. The results clearly show   absence of non-random occurrence of different genotypic combinations in all   the populations studied. The occurrence of all possible genotypic   combinations indicates enough frequency of crossing over among these enzyme   loci. The absence of linkage disequilibrium in this study thus indicates   that selection did not play any role and free recombination among the genes   resulted random occurrence of all combinations

Complete absence of linkage disequilibrium between enzyme loci in natural populations of Drosophila ananassae