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To determine which of several gamma-irradiated mutants of amaranth Ficha   cultivar and K-433 hybrid are most genetically similar to their   non-irradiated control genotypes, we performed amplified fragment length   polymorphism (AFLP) based analysis. A total of 40 selective primer   combinations were used in reported analyses. First analyses of   gamma-irradiated amaranth mutant lines were done used the AFLP. In the study,   primers with the differentiation ability for all analysed mutant lines are   reported. The very specific changes in the mutant lines´ non-coding regions   based on AFLP length polymorphism were analysed. Mutant lines of the Ficha   cultivar (C15, C26, C27, C82, C236) shared a genetic dissimilarity of 0,11   and their ISSR profiles are more similar to the Ficha than those of K-433   hybrid mutant lines. The K-433 mutant lines (D54, D279, D282) shared genetic   dissimilarity of 0,534 but are more distinct to their control plant as a   whole, as those of the Ficha mutant lines. Different AFLP fingerprints   patters of the mutant lines when compared to the Ficha cultivar and K-433   hybrid AFLP profiles may be a consequence of the complex response of the   intergenic space of mutant lines to the gamma-radiance. Although a genetic   polymorphism was detected within accessions, the AFLP markers successfully   identified all the accessions. The AFLP results are discussed by a   combination of biochemical characteristics of mutant lines and their control   genotypes

Using of AFLP to evaluate gamma-irradiated amaranth mutants