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Objective: The aim of this study was to evaluate the effect of Aloe vera gel on sperm DNA integrity in rats. Materials and Methods: This research used completely random design using 24 male rats (Rattus novergicus) weighing 210–220 g. The rats were divided into four groups with six rats in each group. Group 0 (T0) was treated with distilled water as placebo. Group 1 (T1) was given with 200 mg/kg body weight A. vera gel, Group 2 (T2) was given with 300 mg/kg body weight A. vera gel, while Group 3 (T3) was treated with 400 mg/kg body weight A. vera gel. A. vera gel was given every day for 21 days orally to evaluate the effect on sperm DNA integrity. Results: The rat administration of A. vera gel with a dose of 400 mg/kg body weight significantly increases on the sperm DNA damage. Conclusions: The finding of this research indicates that A. vera gel affected the DNA integrity of spermatozoa, and its use is to be restricted in male animals, especially those used for breeding.

SPERM DNA INTEGRITY OF THE RAT TREATED WITH DIFFERENT CONCENTRATION OF ALOE VERA GEL EXTRACT