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An isolation and identification of phenolic compounds from Halban Leaves (Vitex pinnata Linn), a traditional medicinal plant in Aceh, has been done. Halban leaves were extracted through methanol maceration followed by isolation. The methanol extract went into n-hexane and ethyl acetate partition consecutively resulting in 3 different fractions of ethyl acetate and n-hexane extract. The ethyl acetate fraction demonstrated the most promising antioxidant and cytotoxic activity. Therefore, purification of ethyl acetate fraction was done by column chromatography [SiO2; (i) nhexane-ethyl acetate 10:1, 1:1 ; (ii) n-hexane-ethyl acetate = 2 : 1); (iii) n-hexaneacetone = 1:1]. The pure isolate as identified by using UV, IR, NMR, and MS spectra afforded 2 phenolic compounds, namely (1) 4-hydroxymethyl benzoate and (2) phydroxymethyl benzoic acid (PHBA). The antioxidant activity of compound 1 and cytotoxicity activity of 2 expressed in IC50 and LD50 was 41.08 ppm and 59.41 ppm, respectively.

Isolation and Antioxidant Activity of Phenolic Compounds from Halban Leaves (Vitex pinnata Linn) in Aceh