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A sensitive HPLC method for direct determination of allantoin in ruminal digesta, urine and blood serum of sheep is described. Blood serum and urine samples were diluted 1:3 with deionized water. Separation and quantification of allantoin was achieved using three long Nova Pac C 18 -co- lumns (Waters) and UV detection at 205 and 215 nm. The low coefficient of variation and satisfac- tory recovery of allantoin added to assayed samples (~100%) provide a suitable tool for routine quantification of allantoin in a large number of urine, ruminal digesta and blood serum samples.

A highly efficient method for determination of allantoin in ruminal digesta, ovine urine and blood serum by HPLC