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The objective of this study was to investigate the effect of linoleic acid-rich plant oils on rumen fermentation parameters. Three experiments were carried out using the batch culture system. Ruminal inoculum was obtained from cannulated sheep, goats and dairy cows. The substrate for in vitro incubations was supplemented in the experimental groups with evening primrose (Oenothera biennis), borage (Borago officinalis) or Saint-Mary thistle (Silybum Marianum) seed oils to 5% in substrate dry matter. High-linoleic oils improved protozoa counts in sheep and cows, and bacteria counts in goat rumens. Changes (P<0.05) of particular volatile fatty acid concentrations were noticed in all animal species. Advantageous changes were found in the level of total C18:1 trans in all species and in t11 C18:1 in goats and cows.

Effect of oils rich in linoleic acid on <i>in vitro</i> rumen fermentation parameters of sheep, goats and dairy cows