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In plant tissue culture, the culture vessels are usually covered tightly with screw caps, aluminium foils, parafilm, or plastic wrap. This condition restricts the exchange of gases in culture vessels and will affect negatively the growth of explants. The use of ventilated closure improves the air quality in culture vessels. Microboxes provided with different types of filter (yellow filter with Kv value 13.09 Gas Exchange (GE)/day, green filter with Kv value 81.35 GE/day and without filter) on their closure were examined as culture vessels for growing rubber explants at multiplication step. The purpose of this research was to observe the plant condition in different types of microbox corresponding to the morphology, stomata and chlorophyll content of the shoots. The results showed no significant difference of shoot height on each microbox. The use of ventilated closure increased significantly new leaf formation and decreased leaf fall. Normal size and color of leaves were found on shoots grown in microbox with green filter. Chlorophyll analysis revealed no significant differences on three types of microbox, however visual observation showed that leaves were greener on microbox with green filter. The stomata condition of shoots on microbox with green filter were similar with those of mother plants in green house, while different condition of stomata were found on shoots grown in microbox with yellow filter or without filter. In normal environment such as at the field and green house, most of stomata were closed, in microbox provided with filter on the closure, most of stomata were half open, while on microbox without filter most of stomata were wide open. [

Pengaruh ventilasi terhadap morfologi, stomata dan kadar klorofil tunas karet yang diperbanyak melalui microcutting Influence of ventilation on morphology, stomata and chlorophyll content of Hevea shoots propagated through microcutting