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Summary Somatic embryo culture of tea (Camellia sinensis L.) on an agar-solidified medium consists of embryos of different sizes, colors and developmental stages. One gram of mostly globular somatic embryos were cultured on a solid proliferation medium of WP containing 57.1 µM IAA and 4.4 µM BAP to observe their morphological variations with respect to embryo size, color, and developmental stage over one culture passage of 6 weeks. Fresh weight of somatic embryos increased slowly during the first 4 weeks and then sharply thereafter. At the fourth week, the number of embryos increased considerably although their weight did not increase, indicating the formation of secondary embryos. The average size of tea somatic embryos did not change significantly over the culture period, however, the embryo size was already highly varied at the start and increased as the embryo developed. About one half of the embryos were yellowish and  the rest were divided equally between the greenish and reddish embryos. At the initial culture, 60% of the embryos were at the globular, 30% at heart and 10% at torpedo stage. Generally, globular embryos developed into later-stage embryos as the culture progressed, however, on this proliferation medium almost 80% of the embryos remained at the globular and heart-shaped stages even after the sixth week. If single globular somatic embryos with a particular color were cultured on a solid regeneration medium of WP with 0.47 µM kinetin, 0.69 µM ABA and 0.29 µM GA 3 , some of them especially the yellowish embryos underwent color change. Most of these single globular embryos developed gradually into the later stages. While the initial colors of embryos affected the rate of developmental stage changes, yellowish globular embryos tended to develop more rapidly into cotyledonary or germinant stages than the greenish and reddish embryos. Ringkasan Biak embrio somatik tanaman teh (Camellia sinensis L.) pada medium padat terdiri dari embrio dalam berbagai ukuran, warna dan stadia perkembangan. Satu gram embrio somatik yang sebagian besar dalam stadia globuler telah dibiakkan pada medium padat proliferasi (medium WP dengan IAA 57,1 µM dan BAP 4,4 µM) untuk mengamati keragaman morfologi embrio dalam hal ukuran, warna dan stadia perkembangan dalam satu periode kultur 6 minggu. Berat basah embrio somatik meningkat perlahan pada 4 minggu pertama kemudian meningkat dengan tajam. Pada minggu keempat, jumlah embrio melonjak walaupun beratnya tidak meningkat, hal ini menunjukkan adanya pembentukan embrio sekunder. Ukuran rata-rata embrio somatik tidak berubah secara nyata selama periode kultur, tetapi ukuran embrio sudah sangat beragam sejak awal kultur dan terus meningkat sejalan dengan berkembangnya embrio. Sekitar setengah dari embrio berwarna kuning dan sisanya terdiri dari embrio berwarna hijau dan merah. Pada awal kultur, 60% embrio berada pada stadia globuler, 30% stadia bentuk-hati dan 10% stadia bentuk- torpedo. Pada umumnya embrio globuler berkembang ke stadia lebih lanjut sejalan dengan waktu, tetapi pada medium proliferasi ini hampir 80% embrio masih dalam stadia globuler dan bentuk-hati pada minggu keenam. Apabila embrio somatik globuler tunggal dengan warna tertentu dibiakkan pada medium padat regenerasi (WP dengan kinetin 0,47 µM, ABA 0,69 µM dan GA 3 0,29 µM, sebagian embrio terutama embrio kuning akan mengalami perubahan warna. Sebagian besar embrio globuler tunggal ini berkembang secara bertahap kestadia per- kembangan lebih lanjut. Warna awal embrio berpengaruh terhadap kecepatan perubahan stadia perkembangan embrio, dengan embrio globuler awal warna kuning cenderung lebih cepat berkembang kestadia kotiledon dan kecambah dibandingkan dengan embrio hijau dan merah.

Morphological variations during the development of somatic embryos of tea (Camellia sinensis L.) in vitro Keragaman morfologi selama perkembangan embrio somatik teh (Camellia sinensis L.) in vitro