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Leaf spot and fruit rot of chilli caused by Alternaria alternata (Fr.) Keissler is one of the most destructive disease of chilli (Capsicum annuum L.). Study was undertaken to conduct collection of samples, isolation, identification, pathogenicity, morphological and cultural characters of pathogen on different culture media in vitro. The isolations were made from the symptomatic samples collected from chilli plot from All India Coordinated Research Project on Vegetable Crops, Mahatma Phule Krishi Vidyapeeth, Rahuri. The isolation of fungal pathogen was done from affected samples of chilli leaf. Further purified and maintained on PDA media. Pathogenicity of test fungal pathogen was proved on healthy chilli plant in earthen pots under glasshouse conditions. The pathogen was identified as Alternaria alternata on the basis of morphological and cultural characteristics. The pathogen showed typical symptoms of leaf spot on chili in glasshouse. Alternaria alternata showed spots with concentric rings on leaves. Alternaria causes small reddish purple spots most characteristic symptom was brown to dark spots on older leaves. The circular spots enlarge and soon develop dark, concentric rings or ridges gaved target-like appearance. Lesion growth stopped by major leaf veins. Spotted leaves soon turn yellow, wither and died. In cultural studies, it was observed that the fungal test pathogen Alternaria alternata showed maximum radial growth and excellent sporulation on PDA media.

Morphological and cultural studies of Alternaria alternata causing leaf spot and fruit rot of chilli