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Plant root exudates composition is genotype specific and its expression pattern helps to find the diversity and the role of exudates in plant growth and development. Study of exudates requires standard protocols. During this investigation established non-invasive method of root exudates collection by raising seedlings in a plastic cups filled with silica sand without addition of nutrients externally. Number of seedlings required (4/cup) and right growth stage of seedling to collect sufficient quantity of exudates (9th DAS) for analysis was experimentally determined Spectrometer analysis of root exudates (A280) was made to confirm the presence of biochemicals in a sample prior to undergoing for detailed analysis. For concentrating of root exudates, lyophilizer was found ideal. The possibility of reviving of seedlings whose exudates was analyzed by TLC.

Standardization of a novel method of root exudates collection and analysis of cotton root exudates by thin layer chromatography