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This study was performed according the principles of Good Clinical Practices. The protocol was approved by an ethical committee. After at least 7 days of acclimatization and based on pre-treatment flea retention rates, 48 adult domestic cats (1.97–5.72 kg BW) were allocated to 6 groups: 3 control groups (A, B, C) and 3 DP treated groups (D, E, F). The cats in the treated group were administered 0.9 mL of DP on day 0 while the cats in the control group were untreated. The animals were infested individually with 100 adult fleas (Ctenocephalides felis) on days –5, –2 and 7. The fleas were combed and removed from cats for assessment and categorized as live, moribund or dead: on day-4 for all cats; on day 0: 3 and 5h (A and D), 4 and 8h (B and E), 1 and 2h (C and F) after treatment; on day 7: 1and 4h (A and D), 2 and 6h (B and E), 3 and 12h (C and F) after infestation. Live fleas were considered as a failure and insecticidal efficacy was calculated using arithmetic means (AM) and the Abbott formula. The groups were compared at each time-point posttreatment by one-way ANOVA. The level of significance was set at 5%. The cats were under general health observation for the duration of the study.

british small animal veterinary association ebooks

Onset and speed of kill of a topical administration of a dinotefuran-pyriproxyfen (Vectra® Felis) combination against Ctenocephalides felis fleas on cats