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?- Linolenic acid (GLA) is an intracellular primary metabolite produced by some plants and fungal species via several distinct biosynthetic pathways. It is an essential fatty acid, and is mainly used in treatment of various diseases such as rheumatoid arthritis, multiple sclerosis, schizophrenia, atopic eczema and premenstrual syndrome. It is also a precursor of a number of biologically active compounds. In present work, strains of Cunninghamella echinulata var.elegans MTCC 552, Mucor rouxii MTCC 386, Mucor hiemallis MTCC 1278 and Rhizopus stolnifer MTCC 2591 were screened for maximum production of GLA. Cunninghamella echinulata var. elegans MTCC 552 produced maximum GLA (13.7 ± 0.31 mg/g of DCW), and hence selected for further work. Many crucial factors that affect GLA production were optimized in two steps. In the first step, one factor at a time method was used to investigate the effects of media constituents. Subsequently, in the second step, concentration of media components was optimized using Response Surface Methodology (RSM). These optimizations increased the GLA production to 19.8 ± 0.35 mg/g of DCW.

Media Optimization for the Production of ?- Linolenic Acid by Cunninghamella echinulata var.elegans MTCC 552 Using Response Surface Methodology