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Surface disinfection and germination of propagules, used in inoculants based on arbuscular mycorrhizal fungi (AMF), are a requirement of utmost importance for the successful establishment of symbiosis under in vitro conditions. This study assessed a protocol to establish a method of disinfection and germination of spores and root fragments with vesicles of the AMF Glomus sp. (GEV02) under in vitro conditions. The Glomus sp. (GEV02) spore disinfection was carried out using a combination of several disinfecting agents: chloramine T, hypochlorite, calcium, and antibiotics. For mycorrhized root fragments as a complement to the use of the aforementioned disinfectants, an ultrasound treatment was applied before the disinfection process. In addition, we employed a method of germinating the spores and root fragments mycorrhized with Glomus sp. (GEV02) that used different concentrations of flavonoid 3,5,7,3’,4’-pentahidroxi flavone. The results reflected the positive impact of the disinfectants calcium hypochlorite (1%), chloramine T (2%) + two drops of tween 20, followed by an antibiotic solution containing 200 mg of sulfated streptomycin and 100 mg of sulfated gentamicin, as well as the use of the flavonoid 3,5,7,3’,4’-pentahidroxi flavona (5 μM) during germination, on the percentages of contamination of the infective propagules. This study allowed us to establish a new methodology that ensures the rapid and successful germination of spores and root fragments with vesicles of the arbuscular mycorrhizal fungi, Glomus sp. (GEV02) while successfully avoiding contamination.

ciencia y tecnología agropecuaria

Metodología para la desinfección y germinación de esporas y fragmentos de raíces micorrizados con Glomus sp. (GEV02) para su uso bajo condiciones in vitro