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The blood serum samples from healthy rats and rats with inoculated tumors 14 and 28 days after transplantation of cholangiocarcinoma cells were studied using Raman spectroscopy. It was shown that the amide I band intensity differs in the spectra depending on tumor stage and it correlates with the serum protein concentration. The blood glucose concentration increases in some rats, leading to protein glycation and the amide 1 band change. The band at 1670 cm-1 is most pronounced in the spectra on 28 day of the experiment and may be associated with an increase of β-structural elements in protein conformation. The principal component analysis allows evaluating the differences in Raman spectra of healthy rats and rats on day 28 after tumor transplantation.

Исследование сыворотки крови у крыс с трансплантированной холангиокарциномой с использованием спектроскопии комбинационного рассеяния света