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Testing of spermatozoa fertility in vivo is needed to find out the extent of the ability of spermatozoa fertilization in the female reproductive tract. The fertility ability of spermatozoa in fertilizing the ovum is indicated by the number of fertile eggs from the number of eggs incubated resulting from the mating with Artificial Insemination (AI). The aim of this research is to know the percentage of fertility of eggs from AI using semen which has been stored cold for 8 hours on Ringe's diluent with the addition of antioxidant glutathione. Collection semen with teaser female method is done in Poultry Breeder Installation of Sekolah Tinggi Penyuluhan Pertanian (STPP) Malang. The 30 female chickens were divided into 3 groups of treatment P0: AI using fresh semen with Ringer's diluent, P1: AI using cold cement with Ringer's Diluent (8 hours duration storage) and P2: AI using cold semen with 0.5 mM addition in Diluent Ringe's (8 hours duration storage). Experiment design using Complete Randomized Design (RAL) and  nonparametric data analysis with chi-square. The observed variable was egg fertility calculated based on egg fertility percentage. The results showed that the dilution treatment had a significant effect (P <0,05) on fertility egg, with the best result indicated by P0 that is 76% fertility, followed by P2 with fertility 42% and P1 which showed the lowest fertility was 34%. IB with cold cement has not demonstrated a good percentage of fertility despite 0.05 mM glutathione added as an antioxidant. It is recommended that AI in domestic poultry using fresh semen with Ringe's diluent, is done as soon as possible to obtain optimum fertility.

Fertilitas Spermatozoa Ayam Buras dengan Penambahan Antioksidan Glutathione dalam Pengencer Ringer’s Selama Simpan Dingin