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This study investigated the effect of fibroblast growth factor 2 in monolayer followed by transforming growth factor 1 in 3-dimensional culture on chondrocytic matrix production. Under the culture conditions presented, FGF2 is mitogenic but not chondrogenic. In addition, TGF 1 stimulation produced predominantly type I collagen.

Sequential Use of Fibroblastic Growth Factor 2 and Transforming Growth Factor β1 in Cartilage Tissue Engineering Using Canine Chondrocytes