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Methods of static potentiometry and UV-VIS spectrophotometry were applied for the determination of protonation constants of (10-[4-aminobenzyl(hydroxyl)phosphonylmethyl]-1,4,7,10-tetraazacyclo-1,4,7-triacetic acid) (DOTA(NH2)) and stability constants of holmium and yttrium complexes with DOTA(NH2). The DOTA(NH2) protonation constants of [HA] (log 1 = 12.7); [H2A] (log 2 = 21.4) and [H3A] (log 3 = 27.9) were experimentally determined by the static potentiometry. The stability constants of Ho-DOTA(NH2) (log KHo DOTA(NH2) = 18.0) and Y-DOTA(NH2) (log KY-DOTA(NH2) = 17.8) complexes were determined by UV-VIS spectrophotometry using the competitive reaction of the DOTA(NH2) – metal complexes with Arsenazo III.

the open inorganic chemistry journal

Determination of Protonation Constants and Stability Constants of Lanthanides with Derivative of H4DOTA Complexes by the UV-VIS Spectrophotometry and Potentiometry