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Enhancement of pullulanase production by Raoultella planticola DSMZ 4617 using optimized medium formu- lation was investigated in batch fermentation using 500-mL shake flask. The fermentations were carried out, firstly, to search for a suitable cultivation medium for enzyme production and followed by the evaluations on the influence of car- bon and nitrogen sources and also initial culture pHs on the secretion of pullulanase by this bacterium. The modified min- eral Czapek medium was found suitable to produce substantially high activity of pullulanase (320 times higher) as com- pared to 'Ohba-Ueda' medium. This bacterium was found superior in pullulanase production using sago starch and pep- tone as carbon and nitrogen sources, respectively. Using the optimized medium, the bacterium produced 0.95 U/mL of pullulanase at initial culture pH of 7 and incubation temperature of 30 o C.

Enhancement of Extracellular Pullulanase Production by Raoultella planticola DSMZ 4617 Using Optimized Medium Based on Sago Starch