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Black mulberry (Morusnigra L) has been consumed as fresh as well as processed forms due to its nutritional value. Total phenolics, flavonoids، anthocyanins, antioxidant capacity and major phenolic compounds were identified in mulberry to investigate the health-related constituents of black mulberry products. The results indicated that the mulberry had contained the high amounts of natural antioxidant and antioxidant activity., Mulberry can be mixed with if yoghurt is the most consumed within the wider fermented milk products, mulberry extract at level0%,1%,2%,4%, 6%, 8% and 10% ml to 100 ml fermented milk to provide high nutritional value of yogurt products.. The yogurt sensory evaluation reported that if mulberry is added up to 8 ml to 100 ml milk, yogurt is acceptable. The biological experiment showed that for 5 days/week, the rat control group fed on basal diet and the 3٠ rats were rat group injection with 3.0 ml/kg rat from a testosterone enanthate tank, for 2 consecutive weeks of benign prostatic cancer induction. These rats were divided to control positive diet on basal diet. The 3, 4, 5 and 6 rat groups fed basal diets and were taken separately orally from the mulberry aqueous extract at level 100, 200, 300 and 400 mg/kg body weight/ day for six-week storage period. Prostate weight and index content as TNF-α and. PEG2 were calculated and analyzed, oxidative stress was also calculated. The findings showed that the different groups fed on basal diet and were administered orally separately from the mulberry aqueous extract, prostate weight, prostate index TNFα, PEG2 and oxidative stress were improved over positive regulate. In addition, histological prostate tests were verified with the expected findings, it could inferred that the mulberry had active antioxidant in it. Home Econ. J. Vol. (36), No. (1). 2020 72 INTRODUCTION The findings showed that the different groups fed on basal diet and that prostate cancer were taken separately is one of the most frequently diagnosed malignancies in men worldwide (Siegel et al., 2017). Approximately 70% 80% change too prostate cancer patients experience bone metastasis (Shah et al., 2004), and 5% 12% of prostate cancel patients with c have regional lymph node metastasis. Both cancel cells and the tumor microenvironment contain tumor necrosis factor (TFNA), which has pluripotent effects on tumorigenesis and tumor progression (Liu et al., 1998) prostaglandins (PGs) influence several pathways which have been shown to play a role in carcinogenesis, such as cell proliferation, angiogenesis, apoptosis, and mutagenesis. PGs are derived from liberation of arachidonic acid. Prostatic tissues are nearly 10-fold higher in benign prostatic tissues (Chaudry et al.,1994). PGE2 has also been shown to promote osteoblast cell growth and prostate cancer cells (Tjandra winata et al., 1997). PGE2 interacts with four different receptor subts of E Prostanoid (EP1.EP4), which belong to the superfamily of G Protein-coupled receptors (Breyer et al.,2001). (Pritskhalaishvili et al.,2001) demonstrated that cells of human prostate cancel (PCa) cause the apoptotic death of the most active antigen presenting cells. Dendritic cells (DC), which are responsible for inducing unique immune responses to antitumor. The effect on murine PCa cells RM1 on the survival on the immature and tumor necrosis factor (TNFA) stimulated mature DC has been evaluated. The aim of this investigation was to test natural mulberry antioxidant mulberry extract/100 ml milk at rates 2,4,6, and 8 to generate yoghurt. In addition, the mulberry antioxidant was used as an experimental biological to protect male rats from cancer and was administered orally for six weeks at level 100, 200, 300 and 400ppm /kg /day. MATERIALS AND METHODS Materials Black Mulberry (Morus nigra L.) was collected from local market at GizaEgypt and washed properly under running water to remove foreign particles, further dried in a portable hot air oven at 50 60 ° C. The dried berries were milled using a blender (Crown star model AJ 20 blender) into powder at 1800 rpm for 5 min and kept in sterile containers, stored under room temperature of 25C for analysis. The owder thus obtained was twicewich sieved to eliminate the coarse particle. Kits as TNFα.and PEG2 and oxidative stress (MDA, COT, SOD and GSH) for determination of the parameters were purchased from Sigma Aldrich Corp., Home Econ. J. Vol. (36), No. (1). 2020 73 MO, USA. The cow's milk was purchased from local market, Giza-Egypt, which it was used to prepare the yogurt. Methods: Estimation of total phenolic acids, total flavonoids total anthocyanin compounds The total phenolic content in the mulberry extract was determined with FolinCi ocalteu reagent using the Qawasmeh et al ., ( 2012) test. The measured UV reading was 760 n m. Gallic acid was used as a baseline (1 mg / ml) and the findings were expressed as equivalen ts of gallic acid (GAE mg/100 g of dry mass). The total contentof flavonoids was calculated usi ng the Eghdami and Sadeghi (2010) methods. The absorbance was measured against a blank s olution at 510 nm, and the total content of flavonoids wasexpressed as mg of qu ercetin equivalent per gram of dry weight (mg QE/100 g DW). The total content of anthocyanin (TA) was calculated using the differential pH method (AOAC, 2006). Extract TA was expressed as the equiva lent in milligrams of cyanidine-3-O-glucoside (C3 G) per 100 g of the day. Determination methods of antioxidant activity in mulberry Diammonium salt ABTS (2, 2-Azinobis 3-Ethulbenzothiazoline6-Sulfonic acid) the ABTS assay was conducted using Miller and RiceEvans (1997) and Ramesh Kumar and Sivasudha (2012) methods used for the study 1ml ABTS Reaction solution was applied to the 100 μL sample extract and immediatelyafter 1 minute of initial mixing the absorbance was calculated a t 734 nm The DPPH assay was performed as defined by Ravichandran et al., ( 2012) the abservance of the mixture with the UV.Visible spectrophotometer wa s calculated at.515 nm.The formula below was used to calculate the percentage of scavenging activities; Inhibition percentage (percentage) =[(controlsample)/control] Where, PowerDPPH absorbance, A sample absorbance combination reaction (DPPH). Preparation of yoghurt from mulberry extract 200 ml of milk was added to each of the four glass beakers containing 500 ml. The five glass beakers were lined with aluminum foil, then pasteurized for 20 minutes at 85 ° C and then cooled down to 35oC. The mulberry extract was applied separately to four beak ers at levels 2, 4, 6, 8 and 10 ml to 100 ml of milk and the first beaker without s ignificantly applied as guide, as well as 6 % starter culture. Home Econ. J. Vol. (36), No. (1). 2020 74 Evaluation of sensory properties of yoghurt samples Twenty professional yogurt panelists have carried out sensory assessments of y ogurt supplemented with mulberry extract at levels 2, 4, 6, 8 and 10 ml/100ml o f milk in terms of taste, odor, texture, colour, overall appearance and acceptability according to Obi et al., (2016). Biological experimental Male Wister albino weaning rats (36 rats) were purchased from the National Or ganization for Drug and Control Research, Giza, Egypt, with a weight of 150to160 g .Rats were housed in individual cages with screen bottoms and fed ad libitum on a oneweek basal ac climatization diet, containing casein (20%), maize oil (8%), maize starch (31%) , sucrose (32%), mg cellulose (4%), salt mixture. Experimental rats were fed for 15 days on fat and basal dies and six rats were randomly divided into six groups for each. The 1 main group was given a further 6 weeks of basal diet and considered negative control rats. The five groups of rats were fed with basal diet (30 rats, 6 rats in each group) were 5 days/week injection of 3.0ml/kg from a testosterone enanthate tank. Prostate weight and prostate index. The prostate of each rat was rapidly dissected out and weighed. The prostate index was calculated for each rat by dividing the prostate weight by the body weight (mg/g). Determination of TNF-α.andPEG2 in prostate cancer Homogeneous prostate products were used to prepare nuclear extracts for the de termination of TNFα, using the nuclear extraction device EpiQuikTM (OP0002, EpiGentek, NY, US). Cells of prostate cancer (PC3) were grown to approximat ely 80 percent confluence in 10cm2 dishes. The cells were put the next day in MEM with 0.2 per cent BSA (SigmaAldrich). Over different time periods the ce lls were treated with TGF.β1 (1and 5 ng / mL) Determination of markers of oxidative stress. Portions of the prostate tissue was homogenized with ice cooled phosphate buffered saline (5mM potassium phosphate, pH7.5). The Catalase (CAT) was calculated as per Aebi (1995) rule the Janknegt et al., (2007) calculated superoxide dismutase (SOD). Reduced level of glutathione (GSH) were calculated using chemical method described in Moron et al (1979). The Lipid peroxidation was determined color metrically as malondialdehyde (MDA) by Yoshioka et al (1979). Home Econ. J. Vol. (36), No. (1). 2020 75 Histopathology. Briefly, for preparing sections of 4 μm thickness, the fixed prostatic tissues fro m differentgroups were treated with paraffin technique. This was followed by deparaffinisation, rehydration, and hematoxyl and eosin (H&E) staining. At l east three stained sections were photographed and used to assess prostate gland ular epithelial height using the ImageJ, 1.46a, NIH image analysis software. Statistical analysis The obtained data were exposed to the analysis of variance. Duncan's multiple range tests at (P ≤ 0.05) level was used to compare between means. The analysis was carried out using the ANOVA procedure of the Statistical Analysis System (SAS, 2004). RESULTS AND DISCUSION Antioxidant content and activity from mulberry extract Antioxidant contentas phenolic content, flavonoids compounds and anthocyanin compounds and antioxidant activity as DPPH and ABTS

Study the effect of natural antioxidants in mulberry on prostate cancer in male Wister albino