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Aim of this study is to evaluate the effect of adding different levels of Zinc and Selenium on extender as antioxidants to improve Friesian-bull semen quality after cryopreservation. Semen was collected, pooled, and extended with five treatment extenders, including control without (E1), 0.3 mg/100ml Zn (E2), 0.6 mg/100ml Zn (E3), 0.2 mg/100ml Se (E4), and 0.5 mg/100ml Se (E5). Semen was evaluated for visual parameters after dilution, equilibration, and thawing. Also, semen was evaluated by CASA only after thawing. Results showed that there are no effects of all additives on sperm characteristics after dilution and thawing. Only visual progressive motility percentage increased (P<0.05) post-equilibration by E5. Percentages of acrosome integrity were enhanced by E4 and E5. Semen analysis by CASA revealed that non-motility, and total and head abnormalities percentages were decreased (P<0.05), while vitality, and total and rapid progressive motility percentages were increased (P<0.05) by E5. Dynamic sperm parameters straightness and wobble) were increased (P<0.05) E5. In conclusion, adding selenium at a level of 0.5 mg/100ml to extender of cryopreserved Friesian-bull semen ha beneficial effects on maintaining sperm parameters and improved sperm freezability. This may be used as a tool for improving cryopreserved semen in artificial insemination centers.

Selenium and Zinc as Supplements to Extenders Frozen Semen for Improving Sperm Characteristics during Cryopreservation