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Under laboratory conditions, the LC50 of newly hatched nymphal stage recorded   200 X104 spores/ml and  129 X X104 spores/ml. For last nymphal state the LC50s recoded 214X 104  and 258 X 104 spores/ml. Also, the LC50 of the adult female and male recorded, 233 and 257X104 spores/ml and 279X104 when treated with different concentrations of Destruxin and nano-destruxin, respectively. Also, under laboratory conditions, the LC50s, were significantly decreased when the adult female of grasshopper Hetiracris littoralis treated with nao-destruxin and reached to 153X104  spores/ml. Under semi-field conditions, the percentage of infestations of H. littoralis significantly decreased to  1.0±0.3, 3±0.1, 5±3.0 and 10±2.9 individuals after treated with nano-destruxin in 20, 50, 90 and 120 days, respectively as compared to 15.2±2.9, 39±3.5, 66±9.6 and 98±6.6 individuals in the control.

journal of egyptian academic society for environmental development d, environmental studies/journal of egyptian academic society for environmental development. d, environmental studies

Evaluating toxicity of nano-extracted Destruxin from Metarhizium anisopliae against the grasshopper Hetiracris littoralis in Egypt