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Hydrogen energy is a clean source for liveliness better than fossil fuel that has hazardous effects on the environment and atmosphere. Food wastes and organics in the sewage sludge are a promising sustainable and renewable source for hydrogen production where amalgamation of waste treatment and energy production would be more than one benefit expressed in treatment of organic pollutants and energy generation. Discovering biohydrogen production from industrial wastewater by dark and photo fermentation was the main aim of this paper. The biogas produced was composed of H2 and CO2, and the maximum H2 content was 25.94%. This ratio was achieved at batch configuration system and initial pH 6.2 with starch concentration 15 g/l. Cause of using dark fermentation effluent (DFE) was used as substrate for A Rhodobacter capsulatus strain and a clostridium culture were cultivated to produce hydrogen under different light-dark cycles. Acetic and butyric acids decreased due to first and second photo stages by 21.9% and 4.1 % respectively. Maximum hydrogen yield was 470.9 ml H2/mol VFAs.

Bio-H2 conversion of wastewater via hybrid dark/photo fermentation reactor