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Corresponding author email: Samaheldebaiky@science.tanta.edu.eg Tel.: 002-01005256803 0000-0002-5172-0222 Received 28/12/2019; Accepted 4/5/2020 DOI: 10.21608/ejbo.2020.21630.1422 Edited by: Prof. Dr. Salama A. Ouf, Faculty of Science, Cairo University, Giza 12613, Egypt. ©2020 National Information and Documentation Center (NIDOC) TOTAL ochratoxins (OTs) and aflatoxins (AFs) of Aspergillus piperis were quantitively detected by fluorometric spectroscopy in both fungal biomass (FB) and culture filtrate (CF). OTs were detected with high concentrations; 38˟10-3 and 32˟10-3 μg/g in fungal biomass and culture filtrate of A. piperis respectively, while, AFs recorded lower concentrations; 3.3˟103 and 2.3˟10-3 μg/g. Consequently, different types of OTs produced in FB and CF of A. piperis were identified using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDSPAGE) method. The molecular weights (MWs) of protein bands from SDS-PAGE step revealed the presence of ochratoxin B, ochratoxin α and ochratoxin C (OTB, OTα and OTC) in FB of A. piperis but, the results revealed that, only OTB and OTα were found in the CF. The MWs of OTB, OTα and OTC were found to be 363, 260,430 KDa respectively. Also, these findings were confirmed by western blotting technique using the antibodies of OTB, OTα and OTC. Toxicity of different concentrations of CF of A. piperis was tested in vitro on seed germination of broad bean (Vicia faba).

egyptian journal of botany

Evaluation and Detection of Ochratoxins and Aflatoxins of Aspergillus piperis by Fluorometric Spectroscopy, Gel Electrophoresis and Western Blotting Techniques