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Determining the cyanogenic potential of crop plants using traditional biochemical methods are laborious and time consuming. Presently, the Feigl-Anger paper method is being employed for qualitative analysis of cyanogenic potential of plant samples in a rapid and cost effective manner. To improve this technique from its status of qualitative to a more reliable semi-quantitative assay, the Feigl-Anger Densitometry (FAD) method was developed. This method measures the intensity of blue spots on the Feigl-Anger paper, after its exposure to HCN released from sorghum leaf tissues during a freeze thaw cycle. For colour intensity measurements, a densitometric software was employed. The densitometry results obtained from test samples and standards were compared with corresponding values obtained from quantitative spectrophotometric analysis. The results of FAD in a set of 66 germplasm lines revealed high correspondence with cyanide contents estimated through widely used spectrophotometric method of Lambert (R 2 =0.9107). Further, our method was also validated with known concentrations of KCN standards (R 2 = 0.9661). Hence it was concluded that FAD can be employed to estimate HCN content in sorghum rapidly with acceptable precision in a simple lab setup. The method can be suitable for screening of large number of mutants and segregating plant populations for the selection of low hydrogen cyanide lines in breeding programmes.

Rapid screening assay for precise and reliable estimation of cyanide content in sorghum