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An acetanilide group herbicide Butachlor and the commercial formulation Machete (50% EC) both are tested to the fish Channa punctata (Bloch). The LC50 values for 96 hours are 160 ppb for Butachlor and 356 ppb for Machete. The fish exposed to /5 of LC50 values of 96 hours, for 8 days and biochemical changes due to enzyme related after the exposure are determined. The decrement changes are observed in total glycogen and proteins of tissues including the nucleic acids, Deoxyribonucleic acid (DNA) and Ribose nucleic acid (RNA) whereas the lactate dehydrogenase (LDH) activity was elevated. The biochemical alterations affect the survival of the organisms and will be the pathway as sign of toxicity at tissue level before the effect is at organism level causing acute toxicity.

Biochemical Changes Induced by Butachlor and Machete 50% EC to the Freshwater Fish Channa punctata (Bloch)