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Production of taro [ Colocasia esculenta  (L.) Schott], a tropical root crop, is declining in many areas of the world as a result of the spread of diseases such as Taro leaf blight (TLB). Taro cv. Bun Long was transformed through  Agrobacterium tumefaciens  with the oxalate oxidase (OxO) gene  gf2.8  from wheat ( Triticum aestivum ). Insertion of this gene was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. One independent transformed line contained one gene insertion (g5), whereas a second independent line contained four copies of the gene. Reverse transcriptase PCR (RT-PCR) confirmed the expression of this gene in line g5. Histochemical analysis of the enzyme oxalate oxidase confirmed its activity increased in the leaves of line g5. A bioassay for resistance to TLB used zoospores of  Phytophthora colocasiae  to inoculate tissue-cultured plantlets. Transgenic line g5 showed the complete arrest of this disease; in contrast, the pathogen killed non-transformed plants by 12 days after inoculation. A second bioassay, in which spores of  P. colocasiae  were inoculated onto disks of leaves of one-year-old potted plants, confirmed that transgenic line g5 had greatly increased resistance to this pathogen. This is the first report to demonstrate that genetic transformation of a crop species with an OxO gene could confer increased resistance to a pathogen ( P. colocasiae ) that does not secrete oxalic acid (OA).

Taro (Colocasia esculenta) Transformed with a Wheat Oxalate Oxidase Gene for Improved Resistance to Taro Pathogen Phytophthora colocasiae