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Intergenomic F 1  hybrids between  L. auratum  and  L. henryi  were made and backcrossed to Oriental hybrids to produce BC 1  progenies. The F 1  intergenomic hybrids produced a relevant frequency of 2n-gamete and fertile pollen. Analyses of pollen size, viability, and germination ability were checked under microscopic observation. GISH analysis confirmed that F 1  intergenomic hybrids possess a set of chromosomes from both parents and showed an intermediate morphological phenotype. Twenty BC 1  plants were generated by in vitro embryo rescue technique, and analyzed for genome composition by GISH. All plants were triploid, showing 12 from female parent (2× Oriental) and 24 from male (2× F 1  hybrid). Based on the GISH analyses, 19 BC 1  plants were derived from FDR 2n-gamete and only one plant was derived from IMR 2n-gamete of F 1  hybrid. The genome composition of 19 BC 1  plants from FDR 2n-gamete possess 12 Orientals + 12  L. auratum  + 12  L. henryi  chromosomes with some extent of homoeologous recombination between  L. auratum  and  L. henryi . However, one plant from IMR 2n-gamete origin contains an odd number of parental chromosomes from F 1  hybrid, showing 12 Oriental + 14  L. auratum  + 10  L. henryi  chromosomes. In this case, two  L. auratum  chromosomes recombinant with  L. henryi  chromosome segments were added and  L. henryi  counterpart chromosomes were deleted, respectively.

(240) Cytological Analyses of BC1 Hybrids Derived from IMR 2n Gamete of Interspecific Hybrid Between L. auratum and L. henryi