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An inexpensive ultrasonic fogging system is presented that aids in the establishment of tissue culture shoots in soil under greenhouse conditions. In addition, ultrasonic fogging may be coupled to CO 2  nutrient enhancement via bubbling CO 2  into the water reservoirs prior to fogging to improve growth and morphogenesis responses of shoots. A list and cost of items for the system and its assembly is given. Transplanted tissue culture shoots of basil ( Ocimum basilicum  L.), hosta ( Hosta sp .), mint ( Mentha sp .), and thyme ( Thymus vulgaris  L.) were tested with this fogging system with and without CO 2  nutrient elevation and compared to the growth of shoots grown under a misting system with and without CO 2  nutrient elevation. In all cases, ultrasonic fogging enhanced survival rates, growth (fresh weights) and morphogenesis (axially shoots, leaves and roots) vs. that occurring in the misting system. For example, thyme and mint shoots exhibited 2- and 5-fold increases, respectively, in fresh weights under ultrasonic fogging with CO 2  compared to misting systems with CO 2 . Associated with enhanced survival and morphogenesis was an overall enhancement of shoot and leaf size and overall maturation responses. This is also reflected in enhanced secondary products obtained from shoots grown under ultrasonic fogging compared to shoots grown in misting systems.

338 CO2-Ultrasonic Fogging System Enhances Tissue Culture Shoot Survival and Growth