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The aim of this work was to evaluate the effect of sucrose on the in vitro muliplication of potato, cultivars Baronesa, Macaca, and Cristal. The nutrient medium used was the MS basal salts and vitamins added to 100 mg·L -1   myo -inositol. Four sucrose concentrations (20, 30, 40, and 50 g·L -1 ) were tested. The pH was adjusted to 5.9 before autoclaving. Each treatment had 15 explants, which were collected from the lower part of the shoot containing two buds. This material was inoculated in a 250-mL flask with 40 mL of nutrient medium. After inoculation the flasks were kept in a growth room under 25 ± 2 °C, 16-h photoperiod, and 19 μMol·m -2 ·s -1  radiation provided by cool-white fluorescent lamps for 30 days. This trial was designed in a randomized block with three replicates. Every 7 days, the parameters were collected as follows: number of buds, shoot length and number of shoots. It was observed that `Baronesa' presented the highest number of buds and rate of multiplication. `Cristal' had a slightly better performance for these parameters. Plants treated with sucrose at 50 g·L -1  led to a higher number of shoots. However, `Macaca' treated with sucrose at 40 g·L -1  had the highest shoot length.

hortscience

326 Quantification of Reduced Sugars, Starch, and Total Soluble Carbohydrates in Potato Plantlets Cultivated in Vitro in Media with Different Sucrose Concentrations