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The susceptibility of third-instar larvae of  Anastrepha ludens  (Loew) to the entomopathogenic nematodes  Steinernema carpocapsae  (Weiser) (All and Tecomán strains),  S. feltiae (Filiipjev), S. glaseri  (Steiner) (NC strain),  S. riobrave  (Cabanillas, Poinar &amp; Raulston), and  Heterorhabditis bacteriophora  Poinar (NC, Patronato, and Tecomán strains), was evaluated under laboratory conditions. Sterile distilled water (1.0 mL) with 4000 infective juvenile nematodes were applied on 300 g of moistened sterile soil into 1000-mL pots, and 20 third-instar larvae were placed on the soil surface, 1 mL of distilled water without nematodes was applied as control. Each nematode treatment was replicated four times. After nematode application, pots were incubated at 25 °C. Mortality of larvae and pupae was evaluated 6 and 12 d after inoculation. Cadavers of larvae and pupae were dissected and examined for the presence of nematodes. Our results showed that Mexican fruit larvae were susceptible to entomopathogenic nematodes.  S. riobrave  and  S. carpocapsae  All strain caused 90% of larval and pupae cumulative mortality,  H. bactetiophora  NC strain and  S. feltiae  killed more than 80%, whereas  H. bacteriophora  Tecomán and  S. glaseri  caused a 52.5% mortality. These results suggest that the nematodes  S. riobrave  and  S. carpocapsae  All strain have a potential as biological control agents against  A. ludens .

009 Susceptibility of Mexican Fruit Fly Larvae to Entomopathogenic Nematodes (Steinernematidae and Heterorhabditidae)