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Bean rust, caused by Uromyces appendiculatus, is a major disease of common bean {Phaseolus vulgaris L.). The most economic and environmentally sustainable method for controlling bean rust is the use of host plant resistance (Mmbaga et aL, 1996). Bulked segregant analysis (Michehnore et al, 1991) is an efficient method to rapidly identify molecular markers linked to a specific gene using bulked DNA from F2 plants. This technique has been used to tag Ur-S and Ur'4 genes, Middle American (MA) and Andean (A) gene pools, respectively, for rust resistance in common bean using RAPD markers (Haley et al, 1993; Miklas et al, 1993). Pyramiding monogenic resistmice genes into a single bean cultivar is a strategy recommended to obtain durable rust resistance (Mmbaga et al, 1996). Molecular markers linked to major genes for rust resistance are usefiil to pyramid these genes into a single cultivar, especially if epistasis is present (Kelly, 1995), Tagging the Ur-? gene from the MA gene pool for specific resistance to rust race 59 with RAPD markers has not been reported. Our objective was to identify RAPD markers linked to the Ur'7 gene using bulked segregant analysis in an F2 segregating population from the common bean cross GN1140 (resistant to race 59) x GN Nebr. #1 (susceptible to race 59).

074 Molecular Markers Linked to the Ur-7 Gene Conferring Specific Resistance to Rust in Common Bean