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Cowpea ( Vigna unguiculata  L.) is an important grain legume, which in developing countries provides much of the protein in human diets. A plant regeneration system for cowpea was developed. Cotyledons were initiated on MS medium containing 15 to 35 mg·L -1  benzylaminopurine (BAP) for 5 to 15 days. For shoot regeneration, the explants were transferred to a medium containing 1 mg·L -1  BAP. Regeneration percentage (1% to 11%) and the number of shoots (4 to 12 shoots per explant) were significantly influenced by genotype. The duration of culturing and BAP concentration in the initiation stage significantly affected the regeneration capacity. Explants initiated on 15 mg·L -1  BAP for 5 days resulted in the highest regeneration percentage. Conversely, the highest number of shoots was obtained from explants initiated on 35 mg·L -1  BAP. This is the first report of plant regeneration of U.S. cowpea cultivars.

Genotypic Response of Cowpea Vigna unguiculata (L.) to In Vitro Regeneration from Cotyledon Explants