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The most appropriate time for VAM inoculation has been assumed to be the early stages of plant growth, and this study aimed to investigate the impact of VAM inoculation at the seedling and at the 10-week geranium plant stage. Seeds were planted in propagation plug trays (200) filled with four different seed-germinating substrates: half were inoculated with  Glomus intraradices  while the other half were noninoculated controls. Two types of media received inoculum or not (control); they consisted of sphagnum peat with perlite in one case and vermiculite in the other. After 10 weeks growth, the root systems were checked for colonization, and height, weight, and leaf number were registered. The subsequent growth was carried out in 4-inch azalea pots with the appropriate medium. The pre-in-oculated plants were transferred in Mycori-Mix, and the controls were transferred in Pro-Mix. All plants received mild liquid fertilization at each irrigation time (20N–2P–20K). The number of cuttings and flowers were monitored 6 weeks after beginning of pot growth. The pots were displayed in a complete factorial block with six replicates per treatment, and 10 plants were used for each experimental unit. Significant differences did occur between plants initiated in the different germinating substrates; when pre-inoculated, 12.5 cuttings and 7 flowers were recorded in comparison to 9.8 and 3.9, respectively, for controls. These results reflect the importance of introducing VAM at the earliest growth stage.

Plant Growth Stage for Geranium Inoculation with Vesicular–Arbuscular Mycorrhizal (VAM) Fungi