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Leaf sections of  Echinacea purpurea  obtained from greenhouse-grown plants were cultured on Murashige and Skoog (MS) medium supplemented with 0.5 to 4.0 μM benzyladenine (BA) and 0.1 to 10.0 μM naphthaleneacetic acid (NAA). The efficiency of adventitious shoot formation from leaf explants varied depending on growth regulator concentrations. About 90% of leaf tissues cultured with 20 μM BA and 0.1 μM NAA produced shoot differentiation. Initially, the adventitious shoot buds were purplish-red in color; they turned to green shoots as young leaves began to unfold. The individual shoots, when excised and subcultured on the MS basal medium containing 10 μM gibberellic acid (GA 3 ), produced 15 to 20 new shoots per culture within 4 weeks.

Shoot Differentiation and Proliferation in Echinacea purpurea through Leaf Tissue Cultures