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Micropropagation of  Vitis bourquiniana  Lenoir `Black Spanish',  V. champini  Planchon `Dog Ridge',  Vitis  hybrids (`Blanc du Bois', `Himrod', and `Niagara Seedless'),  V. rotundifolia  Michx. (`Carlos' and `Dixie'), and  V. vinifera  L. (`Autumn Seedless', `Cabernet Sauvignon', `Carignane', `French Colombard', `Ruby Cabernet', and `Tokay') was accomplished. Shoot tips taken from micropropagated plants in long-term culture were inoculated onto solidified C 2 D medium containing 5 μM benzyladenine. Culture times consisting of either one or two 4-week cycles were compared for effect on shoot number. A range of response among cultivars tested was noted. The best-responding variety was  V. champini  `Dog Ridge', with 5.8 shoots per apex. All other varieties were less prolific. When shoot micropropagation from nodal explants and apices was compared, so significant difference was noted. In vitro micropropagation offers rapid clonal production of grape and is a source of sterile leaf explant material for embryogenic cultures, which, in turn, are useful target for genetic transformation.

Shoot Micropropagation in Vitis as a Source of Leaf-derived Embryogenic Cultures