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Microcuttings of three western black cherry ( Prunus serotina var. virens Ehrh.) phenotypes obtained from seedling trees with profuse or scant root systems were grown in two container sizes to examine the early effects of root constraint. Because manual methods to estimate root length and other characteristics are time consuming and subjective, an image analysis hardware and software system (image capture and analysis system) was used to classify and measure the roots. There was a significant effect of clone on fine-root surface area, coarse : fine root ratio, and root dry weight ( P ≤ 0.05), but root characteristics (profuse or scant root development) of the parent material were absent in the vegetative propagules from these lines. Container size had no significant effect on coarse- or fine-root surface area but did reduce coarse : fine root ratio ( P ≤ 0.05). A threshold effect of container size on root dry weight was detected ( P ≤ 0.1).

Container Size Alters Root Growth of Western Black Cherry as Measured via Image Analysis