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We examined an in vitro culture method for propagating unconditioned, field-grown broccoli(Brassica oleracea L. Botrytis group) from peduncle explants by testing 20 cultivars in fall and spring. Propagation was affected significantly by genotype (cultivar) and season. The percentage of explants regenerating shoots was significantly higher for cultivars grown in spring (17% to 100%) than in fall (0% to 66%). Shoot regeneration from explants of plants within a cultivar also varied significantly (0% to 100% [...]

Utility of in Vitro Propagation for Field-grown Broccoli: Effect of Genotype and Growing Season