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The syrB gene required by Pseudomonas syringae pv. syringae van Hall to produce the phytotoxin syringomycin is activated by plant signal molecules. Extracts from twigs of 12 cherry (Prunus) genotypes were tested for their ability to induce syrB::lacZ fusion in P. syringae pv. syringae strain B3AR132 to determine whether signal activity is correlated with susceptibility to bacterial canker. One-year-old twigs of 'Napoleon', 'Corum', and 12 cherry rootstocks (F12/1, 'Colt', MxM2, MxM39, MxM60, Gi 148-1, Gi 148-9, Gi 154-2, Gi 154-5, Gi 169-15, Gi 172-9, and Gi 173-9) were tested at concentrations of 0.2, 1.0, and 2.0 mg twig dry weight/ml solution for their ability to induce syrB::lacZ fusion, as measured by β-galactosidase activity [...]

Inducing the syrB Gene in Pseudomonas syringae pv. syringae in Twig Extracts from Cherry Genotypes