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Leaf disks of  Rhododendron  cv. `English Roseum' were vacuum infiltrated with buffered solutions of L- or D-phenylalanine. Controls were infiltrated with buffer. Following a 24 hour period under lights (23°C, 16 hr light: 8 hr dark), the disks were cold shocked at 0°C for one hour, then held under the same light regime for three days. Disks were then subjected to a series of cold treatments, thawed, and analyzed for damage visually and by phenolic leakage. Disks infiltrated with D-phenylalanine consistently showed greater damage at warmer temperatures than did disks infiltrated with the L isomer. We believe the inhibitory action of D-phenylalanine upon phenylalanine ammonia-lyase is responsible for this decrease in cold hardiness.

INFLUENCE OF L- OR D-PHENYLALANINE AND SUBLETHAL COLD STRESS ON RHODODENDRON LEAF DISK HARDINESS