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The study was carried out to examine the appropriate media, explant sources, and suitable growth regulators for somatic embryogenesis to establish a rapid mass production system via somatic embryogenesis in  Oenanthe  stolonifera DC. Modified MS media containing higher concentrations of NO 3 -N were more effective for the formation and development of the somatic embryos from embryogenic callus. Liquid media were more effective for the production of somatic embryos than solidified media. Immature florets were found to be the most competent explant sources for embryogenic callus formation. 2,4-D at 1mg/l was highly effective for the formation of embryogenic callus but inhibitory for the development and differentiation of somatic embryo. Somatic embryos were developed from the translucent and friable embryogenic callus. Addition of BA promoted the callus growth synefgistically with NAA and 2,4-D, but the production of embryogenic callus was inhibited by BA.

INDUCTION AND MORPHOLOGICAL CHARACTERISTICS ASSOCIATED WITH EMBRYOGENESIS OF OENANTHE STOLONIFERA DC.