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thidiazuron Abstract. Intact apple (Malus domestica Borkh.) buds were examined by magnetic resonance imaging (MRI). MRI did not excite water in unchilled apple buds and could not image it. When chilling was satisfied, images were produced. We interpret this difference to mean that water is in bound and/or structured form in dormant apple leaf buds before the chilling requirement is satisfied. Conversion of bound to free water occurred equally in the low-chilling-requirement cultivar Anna and the high-chilling- requirement cultivar Northern Spy only after 600 and 4000 hours of chilling, respec- tively. It appears that processes involved in satisfying chilling requirement are also converting water in buds from bound to free form. Absence of free water in dormant buds during the winter signifies endodormancy, whereas when the water is in free form, buds are ecodormant. Thidiazuron, a dormancy-break ing agent, applied to par- tially chilled buds is instrumental in converting water to the free form within 24 hours. Summer-dormant buds contain free water, and they could be classified only as para- dormant. Based on proton profiles, ecodormant and paradormant buds cannot be distinguished but endodormant buds can be readily identified.

Bound versus Free Water in Dormant Apple Buds—A Theory for Endodormancy