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Dickey and Joiner (1966) reported Ca deficiency symptoms occurring first on old leaves of heartleaf philodendron (Philodendron scandens ssp. oxycardium C. Koch & H. Sell.) and pothos [Epipremnum aureum (Linden & Andre) Bunt.], with youngest leaves the last to develop symptoms. However, heartleaf philodendron grown in Cadeficient nutrient solution developed symptoms on young shoots and leaves while old leaves were not affected (Hershey and Merritt, 1987). The Ca-deficient plants recovered when Ca was added to the nutrient solution. The study reported here examined Ca deficiency of pothos to determine if symptoms develop first on young leaves, as is typical for higher plants, or on old leaves, as observed by Dickey and Joiner (1966). Fifty terminal shoot cuttings, each with one fully expanded leaf, were rooted under intermittent mist in tap water in a hydroponic propagation system (Hershey, 1989). When well rooted, the plants were transferred to a static solution (0.9 liters/jar) culture system (Hershey and Merritt, 1986), but with polyethylene reservoirs and with a modified aeration manifold (Sadof and Hershey, 1989). Deionized water and reagent-grade chemicals were used for nutrient solutions. Half the plants were grown in Hoagland #1 solution with 90 μM NaFeEDTA instead of Fe tartrate (Hoagland and Arnon, 1950). The remaining plants were grown in the same solution, except Ca(NO3)2 was omitted. Plants were grown in a glasshouse with air at a minimum of 20C and with natural ventilation. After 4 months in the same solution, when the – Ca plants had severe symptoms, 10 were placed in fresh + Ca solution and 10 in a fresh – Ca solution. These plants were grown for another 5 months without solution

Calcium Deficiency Symptoms of Epipremnum aureum