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Herbertia lahue  is a rare native iris species of South Louisiana and is difficult to propagate. Seed and bulbs were evaluated as potential explants for micropropagating  Herbertia lahue . Green and dry seeds of  Herbertia  were chilled at 8°C for 0, 3, 6 and 9 weeks on MS media containing 0.0, 4.9 and 9.8 μM BAP and 0.0, 5.4 and 10.7 μM NAA. Seeds cultured on 4.9 μM BAP plus 5.4 NAA, and 9.8 μM BAP plus 10.7 μM NAA produced healthy shoots with no roots. Cultures with no hormones produced shoots with roots. Germination appears to be a matter of time and not the effect of chilling, age of seed, nor hormones. Bulbs were scaled to 5 and 10 mm and placed on the above media with no chilling. Bulbs scaled to 10 mm resulted in a high rate of contamination. Scaling to a 5 mm explant produced fewer contaminated cultures. Decontaminated bulbs produced healthy shoots across all treatments.

THE INFLUENCE OF EXPLANT SOURCE AND CHILLING ON IN VITRO PROPAGATION OF HERBERTIA LAHUE